Teaching approaches in some school are still concentrating on memorizing. Teachers have to make the learning is meaningful for the students. One of the alternative tools is by using Brain-Based Learning. The purpose of this study is to investigate the effect of Brain-Based Learning on students' concept mastery in learning electric circuit for 8th-grade students. The method used in this research was experimental research. The research design that used is pretest and posttest design. The sample was taken by random sampling in class. Participants were 49 students at one of International Secondary School in Bandung, West Java, Indonesia. Experiment group learns with Brain-Based Learning (N=26) while the control group learning with lectured based learning (N=23). The results of students' concept mastery that learned using Brain-Based Learning is better than students' concept mastery that learned using lectured-based learning. The improvement of students' concept mastery can be noticed by independent t-test with significant 0.003. Based on the analysis of students' concept mastery results, the N-Gain score in experiment group is 0.43 which categorized as a medium improvement while in the control group is 0.25 which categorized as a low improvement. Based on these results showed that Brain-Based Learning can be an alternative tool to improve students' concept mastery significantly.