To describe development and pilot testing of the Test of Adolescent Sexual Knowledge (TASK) developed by the researcher using content recommendations of the National Sexuality Education Standards. TASK development was guided by a systematic process described by Kirby and Mathtec. Pilot testing involved the use of talk-aloud interviews with 10 youth. After revisions the TASK was converted to an electronic version and retested in 132 participants across the United States. Cognitive interview findings supported preliminary evidence of validity and guided reduction of items. Administration of the revised task to 132 youth ages 12-18 found that the standardized Cronbach's was 0.93 for the entire test and took an average of 40 min to complete. Highest levels of knowledge were in the domains of Healthy Relationships and Personal Safety. Lowest levels of knowledge were found in the areas of Puberty and Adolescence and STIs and HIV. The TASK has been reduced to 69 total items. Future research will seek to psychometrically evaluate the reduced-item TASK in larger and diverse populations.