Virtualization technology has been around for many years, and its use is increasingly becoming common in education, in general and in computing fields, in particular. This can be attributed partly to its potential to reduce costs, boost efficiency and overcome limited resources through its virtual applications such as servers, storage devices and networks. However, the question is: "would that be at the cost of the quality of learning?" Besides, the way virtualized environments impact motivation needs further clarification, since it is a neglected area of research. This quasi-experimental study tried to address such questions through investigating the impact of virtualized networking software (OPNET Network Simulator) on students' learning and motivation. 116 undergraduate students enrolled in a computer networking class at one of the universities in Egypt participated in this study, who were then randomly assigned to an experimental group (N= 59), which used the OPNET Network simulator, and a control group (N= 57) which studied the same content via the traditional physical lab. Two instruments; a networking test, and a Motivation Scale were administered to both study groups prior and post the intervention. Results showed that students of experimental group who used OPNET Networking simulator had significantly higher grades and greater motivation levels than those of the control group.