This study examined interdisciplinary vocational educator teams to identify differences in their team learning behaviours and examined whether their team affective reactions could explain these differences. We used a mixed-methods approach comprising a survey of 117 interdisciplinary work teams with 604 members and a qualitative analysis of our observations of the meetings of six of these teams. The team-level cluster analysis to identify differences in team learning behaviours revealed three clusters that differed significantly (p = 0.00) regarding nearly all team learning behaviours. We named these clusters according to their patterns in team learning behaviours: 'very active all-round teams', 'active all-round teams' and 'active teams with high knowledge sharing'. These differences in team learning behaviours could be explained by significant differences in team affective reactions (p < 0.05). Each cluster was represented by two teams whose team meetings (five per team) were audio- and videotaped. The overall findings of this study indicate that team affective reactions are related to team learning behaviours. The results of the qualitative analysis of the observation data provide additional information that not only positive but also negative team affective reactions can stimulate the engagement in team learning behaviours.