The aim of this study is to investigate motor performance of children by age and gender. A total of 160 children (80 girls and 80 boys) participated in this study voluntarily. Children were divided into 4 groups according to their gender (girls and boys) and age (11 and 12 years). One Way ANOVA and LSD tests were used. There were statistically significant differences in height and weight in all groups. Sit and reach, sit-up, 30 meter sprint and bent arm hang tests results showed that there were statistically significant differences between girls and boys in all age groups. There was no statistically significant difference in plate tapping test. According to the results of the flamingo balance test, a statistically significant difference was found between the age groups. The standing long jump results from 12 year old boys and 11 year old girls are more than the 11 year old boys and 12 year old girls. Participants anthropometric and motor performance test results were among the normal values. Physical characteristics and motor performance parameters vary by age and gender.