Background: ways of perceiving evidence by public health managers, practitioners and policymakers is one of the key determinants of evidence uptake. Recent public health policy in Ontario requires programmes to be based on evidence. Therefore, understanding views about evidence in both practice and policy contexts is important to bridge the research-policy-practice gap in public health. Objective and methods: this qualitative descriptive study examined understandings about evidence in Ontario public health units by comparing perspectives from managers and frontline staff across six geographically-diverse units. A secondary qualitative content analysis was used to re-analyse transcripts of focus groups from the Renewal of Public Health Systems (RePHS) research project. Results: similarities and differences were revealed with respect to how public health managers and frontline staff view evidence. Although both managers and frontline staff understand that multiple forms of evidence exist and that these forms must be integrated when making decisions regarding programme development and implementation, frontline staff highlighted the role of practice-based evidence. Both groups named tools and processes that were available to assist their decision making. Frontline staff indicated capacity building as important for supporting evidence use. Both groups noted that leadership could present a challenge to evidence-based programmes if not supportive of the evidence-based solution for public health problems. However, the understanding of leadership differed between frontline staff and managers. Conclusion: findings from this study provide insight into how use of evidence can be promoted and how to better support policy implementation efforts within practice contexts.