We report a survey of 100 preschool teachers from 15 preschools in Singapore using a modified instrument called the Pre-School Level Environment Questionnaire. The aims were to explore differences in actual and preferred perceptions of preschool teachers' school-level environment and to investigate the relationship among preschool teachers' profile, preschool learning environments and job satisfaction. Quantitative data using paired samples t tests indicated that there were significant differences in teachers' actual and preferred perceptions of their preschool learning environments except for the scale of Work Pressure. Logistic regression revealed a positive influence of Resource Adequacy on preschool teachers' job satisfaction.