The purpose of this study was to explore the effect of participation in an outdoor orientation program on first-year college students' relationships with the natural world. The theory of biophilia was used as a lens through which to understand human-nature relationships. Eighty-five first-year college students on a 21-day outdoor orientation program were assessed at the trip's beginning and end using the Kellert-Shorb Biophilic Values Indicator (KSBVI). The instrument provides a "biophilic profile," a measure of how one relates to the natural world on each of nine separate subscales that collectively articulate expression of biophilic values: aesthetic, dominionistic, humanistic, moralistic, naturalistic, negativistic, scientific, symbolic and utilitarian. A repeated measures MANOVA was conducted to assess changes in the KSBVI subscales over time. Paired-sample "t"-tests were run to better understand the source and direction of change on the KSBVI subscale scores. These "t"-tests indicated statistically significant change from the pre to post scores in eight of the nine biophilic values. The KSBVI was shown to possess a level of sensitivity adequate for implementation in a pre-post design. Replication studies are recommended to further validate use of the KSBVI on adventure education programs in a pre-post format.