The purpose of this multistudy report was to adapt the Basic Psychological Need Satisfaction and Frustration Scale (BPNSFS) to the Italian context. Two studies were conducted. In Study 1, we investigated the dimensionality, reliability, and convergent and discriminant validity of the instrument in a sample of 544 participants (males = 41%) from 16 to 35 years old. In Study 2, we replicated the results concerning dimensionality in an independent sample of 502 participants (males = 42%) from 16 to 35 years old. Furthermore, we analyzed measurement invariance across gender. Results of both studies showed that comparing a series of competitive factorial models, the 6-factor model had the best fit to the data, assessing 6 different but related dimensions: autonomy satisfaction, autonomy frustration, relatedness satisfaction, relatedness frustration, competence satisfaction, and competence frustration. Moreover, the reliability and convergent and discriminant validity can be considered adequate. Finally, the BPNSFS was shown to be invariant across gender. In sum, the BPNSFS can be considered a promising instrument in the context of self-determination theory-based research for investigating satisfaction and frustration of the 3 basic needs in Italy.