The aim of this study is to determine Turkish middle school seventh grade students' awareness about the biotic and abiotic factors which constituted nature and to examine the effects of various variables including gender, living place, the mothers' and fathers' educational level and socio-economic status. For this purpose, The Awareness of Nature Scale is used. A total of 427 students living in rural and urban part of Kutahya province participated in the study. The gathered data is analysed by using frequency tables and percentages. Besides, independent sample T-test and Analysis of Variance (ANOVA) are used for examining the effect of various variables. The results revealed that middle school seventh grade students' awareness about biotic and abiotic factors is insufficient. No statistically significant effect for gender is detected. The students who live in the urban areas are found to be more aware of the biotic and abiotic factors when compared to the students who live in rural. Moreover, the students who have educated parents and high family income are found to be more aware of the biotic and abiotic factors.