The aim of this research was to study the effect of organizational culture on job satisfaction among teachers using a structural equation modeling approach. Hofstede's (1980) dimensions of culture (masculinity/femininity, uncertainty avoidance, collectivism/individualism, and power distance) were used to inform the theoretical framework. For this purpose, 187 secondary school teachers, 101 male and 86 female, in Education District 2 of Urmia, Iran, participated in the study and were requested to answer three questionnaires: Hofstede's cultural dimensions questionnaire (Akour 2006), La Guardia et al.'s (2000) basic psychological needs questionnaire, and Saks' (2006) job satisfaction questionnaire. Findings indicated that power distance and masculinity/femininity dimensions had a direct negative and meaningful effect on basic psychological needs. Furthermore, basic psychological needs had a direct positive effect on job satisfaction. Besides, the effect of uncertainty avoidance, and collectivism/individualism on basic psychological needs was not statistically significant. In short, the findings confirmed the role of organizational culture and basic psychological needs in job satisfaction.