This study examines the influence of religious affiliation on lesbian, gay, bisexual, trans, two spirit, queer, and questioning (LGBTQ)-inclusive practices. Using data from a national survey of educators from pre-kindergarten to grade 12, multivariate analyses of variance models were employed in order to test the effects of religious affiliation on several LGBTQ-inclusive outcome measures. Results show that religious affiliation does have a significant impact on the likelihood that educators will (or will not) practice LGBTQ-inclusive education, however, the pathways to such practices vary considerably across religious groupings. Recommendations are suggested in terms of intervention, inclusive teaching practices, visibility, and leadership.