Recently, many universities have started to do their best to enhance their contribution in the scientific publication. Thus, researchers and faculty members have been offered many various financial and promotional incentives for publishing their papers in international journals, particularly the ISI ones. The present study aims to identify the opportunities and obstacles that faculty members at Najran University can obtain by publishing in ISI journals. In addition, distinction of the most important obstacles that can hinder such publication was another aim of the present study. Based on intensive review of related literature and a series of structured interviews, a questionnaire was developed, validated and verified. A stratified sampling method was used to choose participants of this study. Findings revealed that the contribution of ISI Journals to the identification of globally well-known journals, the development of participants' skills of refereed scientific publication in international journals, and the provision of positive attitudes towards scientific publication were the most important opportunities researchers have got. However, high standards of ISI journals, mastery of foreign languages, mainly English, and the originality of high intellectual topics were the most important barriers or obstacles that faculty members encountered when trying to publish their article papers in ISI journals. No statistically significant differences due to study variables regarding the opportunities were found. Nevertheless, significant differences due to specialization and previous knowledge of publication in ISI Journals were found.