Online research guides offer librarians a way to provide digital researchers with point-of-need support. If these guides are to support student learning well, it is critical that they provide an effective user experience. This article details the results of an exploratory comparison study that tested three key user interface options in LibGuides 2--number of columns, placement of the navigation menu, and visual integration with the library website--to understand their impact on guide usability. In addition to informing our own design choices, our findings can serve as the basis for further investigation into the connections between student learning and the usability of the LibGuides user interface.