The current study extracted, first, 17 general patterns of intonation. Additionally, the present study investigated the roles that might be played by the gender of Ilami EFL teachers in observing the 17 patterns of intonation. Finally, the study determined if intonation as a suprasegmental feature of speech is affected by the gender of Ilami EFL teachers. The population of the study encompassed all male and female EFL teachers in Ilam city from which 40 teachers (20 males and 20 females) were surveyed on interviews for their observing of 17 general patterns of intonation. For inferential statistics, Chi-Square tests were conducted to analyze the data. Statistical analysis was run using Statistical Package for Social Sciences (SPSS). Accordingly, male teachers outperformed female teachers on observing the rules of intonation.