Research in the series of second language learning certify that both cognitive and affective factors within a language learner affect the level of success that will be achieved in learning a second language. This study was an attempt to investigate the relationship among three factors of anxiety, motivation, and autonomy. To this purpose, 207 students were invited from Golestan high school in Ramian, in the north of Iran. Their age ranged from 18 to 22 years. In one session they filled out the anxiety questionnaire, in the next session they completed the motivation questionnaire, and in the third session they answered the items in the autonomy questionnaire. At the end of the term their final scores were recorded. Analysis of the results showed that there was a meaningful relationship between anxiety and motivation, but there was no relationship between anxiety and autonomy, and no relationship autonomy and motivation. Both high-stakeholders and low-stakeholder may benefit from the findings of this study.