The present study aimed at determining high school students' attitudes towards smart board use in biology classes. The study was carried out using the survey model. The study group was made up of 200 high school students. As the data collection tool, the "Student Attitude Scale for Smart Board Use" developed by Elaziz was used. The reliability coefficient of the scale was 0.78, and it was calculated as 0.87 in the present study. The results of data analyses revealed that the students demonstrated positive attitudes in general. Consequently, it could be stated that smart board use in biology classes allows understanding of subjects more easily and rapidly, avoiding time consumption and increasing students' motivation and interest via visual elements. The students' attitude scores did not differ statistically with respect to the variables of "gender" and "smart board use time."