The purpose of the study was to investigate music education and music therapy majors' use of Twitter and their perceptions and knowledge related to policies and practices. Music majors (N = 238) from five universities in the Southeastern and Midwestern United States participated in a 16-question researcher-designed survey. Results indicated that Twitter was most often used for social purposes. Academic use of Twitter was reported less frequently. Music education majors were likely to have knowledge of Twitter-based incidences of unprofessional content. Similar Twitter usage was reported in terms of gender. Student/faculty classroom interactions involving Twitter were minimal. Implications and avenues for possible future research are discussed.