The observation of living animals in school laboratories provides authentic views of biological research. Various studies stress the importance of primary experiences in biology classes. However, educational films may serve as an alternative in some cases. The aim of this study was to investigate student motivation before and after treatments, including (1) an educational film, (2) a hands-on activity with living animals accompanied by an educational film and (3) a hands-on activity with living animals. We investigated the influence of teaching method, gender and class level on student motivation and learning enjoyment. In all treatments, "Temnothorax" ants were addressed, which can be easily kept and observed. This paper is within the scope of a longitudinal project based on the concept of "ant research" in class. Students (N = 140) from six classes (lower and intermediate secondary school levels) participated in the study. Student motivation was measured using a two-stage test design, including a standardised questionnaire on current motivation and a test on learning enjoyment. The findings of this study suggest that the younger students prefer hands-on activities, while students at an intermediate secondary school level are more interested in video-based learning. Since the combination of hands-on activity and video yielded the best results, the potential of using videos as facilitators for hands-on learning is discussed.