Anxiety disorders are the most common mental, emotional and behavioral problem that occurs during exam. University students suffer to some levels of study anxiety, such as; have new experiences, environment and situation. Study anxiety is a real phenomenon. The prevalence of study anxiety has been acknowledged by students. An attempt has been made to assess and compare the level of Anxiety of 25 male students & 25 female students from the Aligarh Muslim University in Uttar Pradesh. The Purpose of the study was to assess the level of anxiety of the two Genders undergoing (UGC NET EXAM) in Physical education. The data was collected by administering "Sinha's Anxiety scale" (SAC), to find out the level of anxiety of two genders the Data was analyzed by applying descriptive statistic i.e., Chi-square (x2), Mean, Standard deviation & t-test. The random sampling technique is used. Results revealed that the two genders students had low level anxiety during examination. After analysis of data it was found that there is no significant difference on level of Anxiety between the two genders students. The level of significance was set at 0.05.