To ascertain the current status of Holocaust knowledge and attitudes of prospective teachers and to inform the development of a web-based educational resource for teachers of the Holocaust, an exploratory analysis was conducted at a public university in Florida. Data were obtained from prospective teachers (N = 464) who completed a knowledge test, a survey related to bias toward traditionally marginalized groups, and a multicultural affinity scale. Statistical analyses were conducted to examine potential group differences for gender, race, age, and college major. No statistically significant differences were found for the knowledge test or students' bias toward marginalized groups. On the multicultural affinity scale, statistically significant results were obtained for gender and race. Results from this study can serve to guide the curriculum of teacher education programs as well as the development of resources such as the website, Teacher's Guide to the Holocaust.