The present study has been carried out to investigate the human barriers of developing virtual majors at Isfahan University; therefore, considering its objective, it is a functional research. It was conducted in combined (quantitative-qualitative) manner via descriptive survey method. In order to do the research, investigating the texts, interview and questionnaire were used. The statistical population to be interviewed was 43 heads of department and the selection of managers and experts in the field of virtual education and information technology at Isfahan University; and the interview was conducted with 13 people. Afterwards, according to the results of interviews and studied resources, a researcher-made questionnaire was provided. The statistical population of the questionnaire was 420 faculty members at University of Isfahan from whom 201 individuals were selected based on Morgan table and Cochran formula as the sample. The results of the data from the interview and the findings from the questionnaires were sequentially analyzed through categorization method and statistical methods via SPSS software. The results showed that the major barriers in the field of human resources there were high amount of work of the professors in attending parts and university responsibilities and weakness in relationship and interaction of the professor and student.