Electronic textbook usage is increasing within higher education while use of traditional textbooks is declining. While some research concerning student preference for textbook type provides insight into student choices, there is a gap in the literature regarding use of electronic textbooks within dental assisting programs. The purpose of this study was to explore dental assisting students' preferences regarding electronic textbook usage during their professional education. Two questionnaires were administered to a group of dental assisting students to ascertain their preferences. One questionnaire was given during the Dental Assisting Program orientation at the beginning of the academic year. The second questionnaire was administered at the conclusion of a nine-month dental assisting curriculum in which students were required to use electronic textbooks for approximately half of their courses. Results from the questionnaires indicated that while students were open to the idea of electronic textbooks and willing to use them prior to beginning in the Dental Assisting Program, at the conclusion of the Program they overwhelmingly preferred to use traditional print textbooks.