The U.S. Department of Education is committed to supporting innovative advances in educational technology to improve teaching and learning across the nation's education systems and to support educators as they incorporate emerging technology into their learning communities. Artificial Intelligence (AI) in education is a complex and rapidly expanding topic because individuals have different knowledge, opinions, and perspectives on using AI, which has implications for all members of school communities. This user-friendly toolkit was developed through engagement with educators, community members and technology leaders and is designed to help educational leaders make critical decisions about incorporating AI applications into student learning and the instructional core. This document connects broad ideas about AI to the establishment of school and district use policies that will guide its effective implementation. This toolkit provides guidance for the effective use and integration of AI in teaching and learning and presents an overview of Federal laws and considerations that are essential to anchoring and ensuring the use of AI in a safe, secure, and non-discriminatory manner across ten key modules--from federal policies to pressing educational issues, including privacy, data security, civil rights, and digital equity. Finally, the toolkit promotes the principles of transparency and awareness in the use of AI in schools, and emphasizes the importance of providing students, teachers, and parents opportunities to opt out of AI-enabled applications in school.