Due to the COVID-19 pandemic, the incorporation of Virtual Learning Environment (VLE) into educational settings has become a universal trend. This study shed light on the new urge to focus on E-learning which serves a fundamental role in language teaching and learning not only in Iran but also in all countries. To help readers surmise the meanings of the key terms, the experimental group of the study received treatment on one advanced reading comprehension passage through textual glosses where the definition of the keywords was available in the margins of the text, followed by the administration of the five multiple-choice tests. The control group received the same passage and tests, albeit without glosses. The results of the experiment revealed that the experimental group of the study outperformed the control group. More specifically, this study explored the positive relationship between the utilization of textual glosses through a VLE on the reading comprehension of the Iranian upper-intermediate EFL MA candidates. The findings of the present study might be used in any educational setting to improve learners' reading comprehension abilities.