The purpose of this research is to examine the effect of sports participation and physical activity on negative emotions and the quality of life of COVID-19. Data were collected through the personal information form, the International Physical Activity Questionnaire-Short Form, the COV19-Impact on Quality of Life scale, and Positive-Negative Affect Schedule (PANAS). Data were collected through stratified sampling from Recep Tayyip Erdogan University and Kirsehir Ahi Evran University. The sample of the study consisted of 888 students. The obtained data were analyzed using SPSS 26.0. Necessary assumptions were ensured before the analysis. Afterwards, descriptive statistics and MANOVA were used in the research. It has seen that the number of students who do not do sports under license, who passively participate in sports 2-3 times a week, and whose physical activity level is moderate are more in number. The findings show that students who do sports under license, passively participate in sports, and are physically active experienced lower levels of negative emotions and COVID-19 affected their quality of life at lower levels. As a result, it can be said that participation in sports and physical activity have an impact on negative emotions and the quality of life of COVID-19 in the sample of university students.