This study aims to find out the extent to which students attending the English Language Teaching Programme (ELT) at Ondokuz Mayis University are aware of the major spelling, vocabulary, and pronunciation differences between American and British English which constitute the most commonly used varieties of English. To this end, 42 randomly selected undergraduate ELT students were administered a short test consisting of two parts; one on the written differences of lexical items and the other one on the pronunciation differences. Just before the test, each participant was asked whether they preferred British English, American English, or a mix of them. The analysis of the answers given for this pre-question shows that 22 participants reported British English, 11 participants American English, and the remaining 9 participants a mix of the two. In the first part of the test, the participants were asked to tick and identify the British and American versions of the provided 15 written entries like the couples of apologise-apologize, color-colour and elevator-lift. In the second part, the students were asked to pronounce the provided 10 words like advertisement, schedule, and can't to a voice recorder.The findings yielded by this current study clearly suggest that the participants are not totally aware of the differences at spelling, word choice, and pronunciation levels between American and British English. We strongly suggest that more time and effort be allocated to the consolidation of the major differences between different varieties of English under courses like Vocabulary and Listening and Pronunciation in the ELT curriculum, thus raising the awareness of learners and prospective English language teachers concerning the presence of different varieties and their distinctive aspects.