This paper takes the undergraduate course "The Introduction of Educational Technology" as an example, and carries out the practice based on the application of Learn Mode. In Taiwan, there were plenty of attempts for the implementation of mobile learning on both elementary and high schools; yet, it has not been extended to the higher education level. As the partnership of the Faith Hope & Love Foundation, HTC Corporation, we made use of the Learn Mode application and conducted the first practice of mobile learning in university. In this study, a design-based research model and the instructional design of the Learn Mode class activities were both pre-developed before the formal implementation. The questionnaires were empirically tested against data collected from 71 undergraduate students. We surveyed the relationship between user satisfaction (via classroom management, software, hardware) and acceptance (via Technology Acceptance Model) for mobile learning, figuring out the enhancement of motivation of learning as well. Through designing and developing class activities with the functions of Learn Mode application, we provide some mobile learning references and suggestions for other higher education institutions and universities. [This work is partially supported by the grant from the Faith Hope & Love Foundation, HTC Corporation, and the grants from the Educational Technology Department of Tamkang University, Tamsui, New Taipei City, Taiwan. For the complete proceedings, see ED557181.]