This article examines particular aspects of higher education during the late Korean Choson period with descriptive analysis. The context of Roman Catholicism and Protestantism between the early 18th and the late 19th centuries is presented. Also examined is Christian higher education during these two centuries. The impact of Christianity on Korean higher education concludes the discussion. The research result is that Christianity has played a significant role not only in the development of religious schools but also in the building of organizational culture in contemporary Korean higher education. Since the traditional Confucian ethics and values which stressed ethical obligations were essentially the main factors of social organizational culture, Christian missionary educators did not eliminate Confucian norms and traditions at their institutes. They attempted to harmonize the values of Christianity and Confucianism. Confucianism has not established Confucian higher education institutions, and therefore Christianity as a representative religion has led the private colleges and universities in current Korean higher education. Therefore, the author concludes that the characteristics of organizational culture in Christian higher education were formal authoritarianism and traditional collectivism based on Confucian moral norms, as well as open democratism and Western individualism based on Christian paradigms. These two paradigms have become the most significant factors that dominate organizational culture in current Korean higher education.