In 2010, the Office of Autism Research Coordination (OARC) and Acclaro Research Solutions, Inc., on behalf of the Interagency Autism Coordinating Committee (IACC), conducted a comprehensive analysis of the 2009 autism spectrum disorder (ASD) research portfolio of major Federal agencies and private organizations. This is the second annual analysis of this nature, following the "2008 IACC Portfolio Analysis Report" released in 2009. The intent of these analyses is to better inform the IACC and interested stakeholders about the funding landscape and current directions for ASD research. Additionally, this analysis examines the extent to which current funding and research topics align with the "2010 IACC Strategic Plan for ASD Research". The "2009 Portfolio Analysis" can also be used by Federal agencies and private research organizations to help guide future funding priorities by outlining current gaps and opportunities in ASD research, as well as serving to highlight current activities and research progress. Findings reveal that total funding for ASD research from all participating agencies and organizations increased by 41% between 2008 ($222,215,342) and 2009 ($314,385,374). This is the result of a 65% increase in Federal spending ($93,701,880) and a 2% decrease ($1,531,848) in private funding between 2008 and 2009. NIH-ARRA funds provided a substantial boost to ASD research funding in 2009, but even without ARRA funding, total Federal investment increased 21% from 2008 ($143,724,845) to 2009 ($173,457,733). Of the 995 ASD research projects funded in 2009, $123,574,025 (39%) and 348 (35%) of those projects were newly established. Appended are: (1) ASD Research Progress on "IACC Strategic Plan Objectives"; (2) Interagency Autism Coordinating Committee Member Roster; and (3) Office of Autism Research Coordination Staff Roster. (Contains 3 tables, 8 figures and 2 footnotes.) [This report was prepared by the Office of Autism Research Coordination and Acclaro Research Solutions, Inc.]