Plant tissue culture has developed into a valid botanical discipline and is considered a key area of biotechnology, but it has not been a key component of the science curriculum because of the expensive and technical nature of research in this area. This manual presents a number of activities that are relatively easy to prepare and perform. The activities also provide a problem-solving approach to learning as a means of conveying basic content and science process skills. Units include: (1) The Process of Science; (2) Aseptic Technique; (3) Culture Methods; (4) Regeneration; (5) Preparation of Tissue Culture Media; and (6) Cell Culture Applications. Appendices include instructor's notes, a supplies directory, case study discussions, statistical tables, and student investigations. (WRM)