A meta-analysis was performed to evaluate the possible association between maternal alcohol drinking during pregnancy and autism spectrum disorder (ASD) in offspring. Twelve datasets from five observational studies were included. Results showed that maternal alcohol drinking during pregnancy was not independently associated with ASD (risk ratio: 0.90, 95% confidence interval: 0.78 to 1.04, p = 0.16, I[superscript 2] = 0%). The results were consistent for heavy alcohol drinking versus no alcohol drinking during pregnancy, and for studies evaluating the influence of maternal alcohol consumption during different trimesters. In conclusion, current evidence does not support that maternal alcohol consumption during pregnancy is independently associated with ASD in offspring.