Martha's Vineyard Sign Language (MVSL) is an extinct village sign language hypothesized to be a sister of British Sign Language (BSL) and a significant contributor to early American Sign Language (ASL) (Groce 1985). After the last deaf MVSL signer died, signs were elicited from five hearing signers. This study analyzes that data through a series of lexicostatistical comparisons following methodology from Woodward (1978) and Guerra Currie, Meier, and Walters (2002). The results show that a sample of 711 MVSL signs is 67 percent similar to ASL, 74 percent similar to Old ASL (OASL), 56 percent similar to Old French Sign Language (OLSF), and 59 percent similar to BSL. Subsequent etymological analysis suggests that most signs shared by ASL and MVSL originated in Old LSF or ASL, and that signs shared with BSL likely came through ASL to MVSL, not the reverse. This suggests that MVSL did not play a large role in shaping early ASL; however, MVSL data may still be useful in reconstructing OASL.