Background: Given the disparity of research regarding Historically Black College and University (HBCU) students' health and chronic disease risk, investigation of exercise readiness and life stress is warranted. Purpose: This study investigated exercise readiness and life stress among undergraduate students at an HBCU. Method: The quantitative cross-sectional study utilized a Stages of Change framework. A survey determined exercise readiness and life stress scores among HBCU undergraduates (n = 304). Results: Most respondents (57%) were exercising regularly and classified in the Action and Maintenance stages. Approximately 44% reported normal Body Mass Index (BMI), and nearly 50% were overweight or obese. Life stress scores ranged from 1 to 31 (M = 14.23, SD = 6.05). Scores were highest in the Precontemplation stage (M = 15.64, SD = 4.31) and lowest in the Contemplation stage (M = 12.54, SD = 5.64). Median life stress scores were not statistically different between exercise readiness groups X[superscript 2] (4) = 55.61, p = 0.193. Discussion: Freshmen, especially females, should be encouraged to continue exercising to maintain a healthy BMI and reduce chronic disease risk. Translation for Health Education Practice: HBCU exercise interventions call for a socio-ecological approach based on current readiness stage distribution.