In August 2010, the Smithsonian Science Education Center (SSEC) received a grant of more than $25 million from the U.S. Department of Education's Investing in Innovation (i3) program for a five-year study to validate its Leadership Assistance for Science Education Reform (LASER) model in three very diverse regions of the United States: rural North Carolina, northern New Mexico, and the Houston Independent School District (HISD). There were a total of 29 multiple choice questions on both the Fall 2011 and Spring 2014 forms of the PASS (PASS-MC) addressing five broad science content standard categories for the elementary cohort and six broad science content standard categories for the middle school cohort. Only students who answered at least one multiple choice achievement question at both time points were included in the analyses for each respective area of analysis. Results for All Regions combined are presented first, followed by the outcomes for the Houston Independent School District (HISD), the New Mexico region, and the North Carolina region. A summary of the Key Findings for each set of analyses is presented at the beginning of each report, followed by information on the samples included, baseline equivalence between the Phase 1 and Phase 2 groups, and the detailed outcomes by grade level (i.e., elementary cohort and middle school cohort) and subgroup.