Disrupting a gene to determine its effect on an organism's phenotype is an indispensable tool in molecular biology. Such techniques are critical for understanding how a gene product contributes to the development and cellular identity of organisms. The explosion of genomic sequencing technologies combined with recent advances in genome-editing…

Cola beverages are widely consumed and popular among students; they can be used as safe reagents to replace toxic chemical dyes. In this study, a safe and improved experiment is described, which successfully demonstrates Beer's law and adsorption process using activated carbon as the adsorbent and cola as the adsorbate. The results of this study…

A straightforward procedure for the synthesis of (Ph[subscript 3]P)[subscript 3]CuCF[subscript 3], a robust, air-stable trifluoromethylating agent, is reported herein. This preparation can be completed in a single 3 h laboratory session and typically provides ample yields (~79--86%) and high purity (>90%). The target complex is characterized by…

Three days into the academic year, the COVID-19 pandemic abruptly led to emergency remote teaching, ERT, of Chemical Experimentation--a revised alternative to the majors' General Chemistry Laboratory at the University of Costa Rica. The course was reoriented to support students' development of nontechnical experimentation skills and scientific…

Teaching through experiments has always been an indispensable teaching method in chemistry and other STEM disciplines. Letting more students have access to experiments at the frontier of scientific research is essential to stimulating students' interest in chemistry. With the advancement of computer technology, virtual simulation experiments…

The aroma volatile compounds in tomatoes are strongly linked to taste and flavor. Commercial tomato varieties, however, generally lack flavor. Tomato breeding programs have therefore started to focus on increasing the flavor-enhancing volatiles, which can be more easily altered than the sugar content. Analytical efforts that identify key flavor…

Course-based Undergraduate Research Experiences (CUREs) are a pedagogical model where a traditional undergraduate course is transformed into a research-intensive course, offering an alternative pathway to research for undergraduate students. Given the novel learning context of CUREs, there is a need to understand the learning that occurs and the…

Magnetic iron oxide nanoparticles were synthesized and stabilized using ammonium cations or poly(vinyl alcohol) to produce amazing materials such as safer aqueous ferrofluids, ferrogels, ferromagnetic inks, plastics, and nanopowders illustrating how versatile materials can be produced just by simple modifications. The synthesis is fast, reliable,…

Here we present a new undergraduate laboratory that will introduce the concepts of time-resolved spectroscopy and provide insight into the natural time scales on which chemical dynamics occur through direct measurement. A quantitative treatment of the acquired data will provide a deeper understanding of the role of quantum mechanics and various…

An advanced undergraduate organic chemistry laboratory experiment involving a rhodium-catalyzed intermolecular hydrocarbon C-H oxidation reaction is described. In the initial phase of this lab, students conduct a C-H amination reaction of ethylbenzene and isolate a benzylic amine product. In the second part of the lab, competition experiments are…

The enrichment of plant foods with gamma-aminobutyric acid (GABA) is currently an interesting issue in the field of nutraceuticals and can be used as an experiment for upper-division undergraduate students. Here, an interdisciplinary hands-on experiment to produce GABA-enriched mung bean sprouts using common household reagents is described. Based…

An iridium(III)-mediated C-H functionalization sequence involving a concerted cyclometalation-deprotonation/migratory insertion pathway is reported for the undergraduate chemistry laboratory. The air- and water-stable iridacycle intermediates are readily isolated and characterized by NMR spectroscopy. Both steps of the experiment are performed at…

A high performance liquid chromatography (HPLC) experiment for the upper level analytical chemistry laboratory is described. The students consider the effect of mobile-phase composition and pH on the retention times of ionizable compounds in order to determine the acid dissociation constant, K[subscript a], of a phenolic acid. Results are analyzed…

In this paper, we propose a simple experimental apparatus that can measure the force variation over time to study the impulse-momentum theorem. In this proposal, a body attached to a rubber string falls freely from rest until it stretches and changes the linear momentum. During that process the force due to the tension on the rubber string is…

Glycoscience continues to be an underrepresented topic in current undergraduate-biochemistry-laboratory curricula. Of the educational laboratories present in this subject area, few introduce students to cutting-edge methods and techniques related to carbohydrates. A multiweek series of experiments is described that highlights a recently published…