Enzyme kinetics is an essential topic in undergraduate Biochemistry courses. A laboratory work that covers the principal basic concepts of enzyme kinetics in steady state is presented. The alkaline phosphatase catalyzed reaction of phenyl-phosphate hydrolysis was studied as a model. The laboratory experience was designed to reinforce the concepts…

Chemical kinetics is a branch of physical chemistry that constitutes an essential part of undergraduate education. In general, a complex and expensive experimental apparatus is indispensable for observing the kinetics of a chemical process owing to the continuity of chemical reactions. Thus, intuitive visualization of the chemical kinetics using a…

Nonlinear chemical reactions produce interesting chemohydrodynamic patterns in an unstirred medium, which act as interesting demonstrations to display the novel phenomena in a nonequilibrium chemical system. Here, we report new outreach experiments: pH chemohydrodynamic patterns modulated by sodium polyacrylate in the bromate-sulfite-ferrocyanide…

We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude…

A Unit Operations Laboratory (UOL) is fundamental to undergraduate chemical engineering education. This article discusses a new course structure for the University of Kansas UOL and details for three new experiments covering the fundamentals of vapor-liquid equilibrium, fluid mechanics, and kinetics. Students apply knowledge from these courses as…

Here we present a new undergraduate laboratory that will introduce the concepts of time-resolved spectroscopy and provide insight into the natural time scales on which chemical dynamics occur through direct measurement. A quantitative treatment of the acquired data will provide a deeper understanding of the role of quantum mechanics and various…

A laboratory experiment that exposes students to fundamental concepts in kinetics and statistical thermodynamics to study systems of great current interest in nanotechnology by employing a foundational technique in physical chemistry is described. A modified commercial flash photolysis spectrometer was used to study photoinduced isomerization…

The effects of kinetic vs thermodynamic control on "endo"/"exo" stereoisomer ratios can be observed in a simple Diels-Alder reaction between "N"-phenylmaleimide and furan. The use of microwave-promoted synthesis affords the cycloadducts in yields ranging from 65-100%, employing reaction times of 1-10 min at…

Quantum dots (QDs) are useful for demonstrating the particle-in-a-box (PIB) model utilized in quantum chemistry, and can readily be applied to a discussion of both thermodynamics and kinetics in an undergraduate laboratory setting. Modifications of existing synthetic procedures were used to create QDs of different sizes and compositions (CdS…

As the need for alternative energy becomes increasingly important, energy research and related industries are rapidly expanding. This lab incorporates current energy-storage research into a second-year lab that instills real-world, industry-relevant knowledge and skills while teaching and reinforcing physical-chemistry concepts. A manganese oxide…

Determination of enzymatic activity is a relevant experiment in teaching chemistry and biochemistry to high school and college students. A method for measuring the kinetic parameters of lactose cleavage by the enzyme lactase is described. This enzyme catalyzes the hydrolysis of lactose to galactose and glucose. The kinetic parameters can be…

First order processes are of paramount importance for chemical kinetics. In a well-established demonstration experiment, the flow of water out of a vertical glass tube through a capillary simulates a chemical first order process. Here, a digital version of this experiment for lecture hall demonstrations is presented. To this end, water flowing out…

An effective way of teaching undergraduates a full complement of research skills is through a multiweek advanced laboratory experiment. Here we outline a comprehensive set of experiments adapted from current primary literature focusing on organic and inorganic synthesis, catalysis, reactivity, and reaction kinetics. The catalyst,…

An experimental protocol that provides an excellent way to discuss concepts at the crossroads of organic chemistry and biochemistry employing biocatalysis is presented. By evaluating several reaction parameters (enzyme source, organic solvent, and acyl donor), it was possible to conduct an enzymatic kinetic resolution experiment using…

A kinetic isotope effect (KIE) experiment is described for the physical chemistry laboratory. Students conduct a hypochlorite (household bleach) oxidation of an equimolar mixture of 1-phenylethanol and 1-deuterio-1-phenylethanol to acetophenone. The reaction occurs in a biphasic reaction mixture and follows first-order kinetics with respect to…