One of the unique contributions of technology in the field of Education is E-learning. The easy accessibility, flexible learning environment, and cost-effectiveness have made e-learning popular among students. Despite its many benefits, several problems have been faced by students, such as a lack of technical knowledge, computer experience, and internet resources, which may decrease their interest in accessing e-learning. In this study, an attempt has been made to examine the attitude of undergraduate students towards e-learning in relation to gender and academic streams. For this, 120 samples were selected from two different colleges using stratified purposive sampling. The Attitude towards e-learning scale developed by Dimpal Rani (2015) was adopted to collect data from the samples. Although the results of descriptive analysis revealed a diverse range of attitudes towards e-learning, the maximum number of students (30%) had a highly unfavorable attitude. The results of the variation analysis explained that there was no significant difference between boys and girls towards e-learning. It was also found that there was no significant difference among the attitudes of Arts, Commerce, and Science students towards e-learning.