Short chain fatty acids (SCFAs) are produced in the gut as a result of microbial action on ingested dietary fiber and have been associated with several health benefits. Herein an undergraduate student experiment that uses gas chromatography equipped with either a flame ionization detector or mass spectrometer for the analysis and quantitation of…

Human anatomy is a key subject area within undergraduate kinesiology programs. However, literature detailing the course structures, available resources, and teaching approaches used to deliver anatomy education in kinesiology programs is lacking. The present study sought to address this deficit by surveying instructors in Canadian university and…

Undergraduates use a spike sorting routine developed in Octave to analyze the spiking activity generated from mechanical stimulation of spines of cockroach legs with the inexpensive SpikerBox amplifier and the free software Audacity. Students learn the procedures involved in handling the cockroaches and recording extracellular action potentials…

While metabolism is a fundamental process in physiology, the measurement of metabolism by living animals often requires specialized and expensive equipment. In 2016, I developed a novel laboratory exercise for animal physiology students where students were able to observe the effects of temperature and long-term temperature acclimation on the…

Regulation is a key concept for understanding the dynamics of metabolism in bacteria. This report outlines a simple laboratory experiment aimed at studying a key form of regulation in bacterial metabolism, exemplifying how microbes switch the flux of carbon towards alternative metabolic fates as a function of nutrient availability and according to…

Flow cytometry has become an important tool in the fields of veterinary, medicine, and biological sciences in general. However, in the introductory and even in advanced university degree courses, students do not have many opportunities to be in contact with this type of technology. This situation motivated us to design a simple and inexpensive…

Teaching introductory anatomy and physiology (A&P) requires a careful balance of conveying core content while at the same time fostering a deep interest in the subject matter. This is especially true in the laboratory where students are expected to not only master experimental procedures but also connect what they learn in the laboratory to…

Most undergraduate students do not have practical experience working with model organisms despite having theoretical knowledge of their importance. This practical was developed to offer students experience working with three species of nematode, including "Caenorhabditis elegans." In the first task, students prepare slides of three…

In this study, we analyzed data of heart rate and pulse amplitude collected by undergraduate students in a physiology lab course during and after the diving reflex. On average, heart rate was reduced 21% at 15 seconds and 29% at 30 seconds during diving. Pulse amplitude generally decreased but with greater individual variation. There is no…

To help educators deliver their physiology laboratory courses remotely, we developed an inexpensive, customizable hardware kit along with freely available teaching resources. We based the course design on four principles that should allow students to conduct insightful experiments on different physiological systems. First, the experimental setup…

The digestive system is a fundamental topic in biological science teaching. Despite its importance, the level of students' knowledge on the topic appears to differ from recommended levels at several educational grades. Their knowledge also seems to be resistant to change; therefore, this discrepancy may be viewed as an epistemological obstacle to…

The California Blackworm ("Lumbriculus variegatus") is a freshwater segmented worm species that has been used by biology instructors as a model system for inquiry-based student investigations. The blackworm dorsal blood vessel pulsation rate is easily quantified. Moreover, this species can facilitate the study of neuromuscular…

Recently, we saw a forced transition in educational practice from traditional methods to online and virtual learning as higher education, along with nearly all aspects of society, was disrupted due to the COVID-19 pandemic. As many of us have returned to face-to-face instruction, questions remain regarding modes of instruction and what forms of…

Failed experiments are a common occurrence in research, yet many undergraduate science laboratories rely on established protocols to ensure students are able to obtain results. While it is logistically challenging to facilitate students' conducting their own experiments in the laboratory, allowing students to "fail" in a safe environment…

A simple bioassay was designed for the detection of adenosine, a human metabolite involved in several physiological processes, on the basis of the functionalization of gold nanoparticles with DNA oligonucleotides selectively binding adenosine. The test was possible thanks to the selective and sensitive binding of adenosine by two DNA…