Promoting students' self-regulated learning (SRL) is one way to improve postsecondary student success. However, few studies have investigated the instructional practices of postsecondary instructors that may support students' SRL. This study sought to fill this gap. An undergraduate mathematics course was observed to determine instruction utilized in classrooms that could influence students' SRL. Results showed that instructor references were made to four areas of SRL: (a) cognition; (b) motivation and affect; (c) behavior; and (d) context. The majority of references concerned cognition and fewer messages addressed motivation. Findings are discussed in terms of postsecondary instructional practices that may foster students' SRL. This project is significant because it developed an observation protocol to assess instructional practices that may support college students' SRL in specific college courses: the Self-Regulated Learning Observation Protocol (SRLOP).