Recent changes due to the COVID-19 pandemic have forced higher education institutions worldwide to transition to online courses. The challenges that the pandemic poses for microteaching are very significant, as it is highly application-based and complicates online implementation. The current study aims to examine prospective teachers' experiences with online microteaching. The participants (N = 21) were Greek university undergraduate students. Specifically, their experiences with online microteaching were examined through reflective texts and follow-up interviews. Data were analyzed based on a mixed-methods approach (quantitative and qualitative) by two independent reviewers. The findings showed that the main advantage of the specific process is the collaboration with fellow students, while the main challenges concern technical difficulties and anxiety. Qualitative analysis revealed that anxiety overlapped with collaboration, while more detailed aspects of the process (e.g., video rehearsals and retakes) provided a unique framework.