In this paper, we discuss the development and initial validation of an instrument, namely IV-SOPRA (Index of Violence Suffered and Practiced), measuring levels of violence suffered and practiced by students in Higher Education institutions; thus, seeking to advance research into the issue of violence connected to Higher Education. Participants are undergraduate students (n = 1188), who responded to an online questionnaire from a university in the south of Brazil. This was a convenience sample. The population was from all undergraduate courses offered at the partner university (i.e. Arts and Humanities; Social Sciences; Sciences; Medicine). Data analysis was conducted using R environment (R Core Team, 2020), and lavaan package (Rosseel, 2011). Confirmatory factor analysis was carried to investigate model fit to the data according to theorised structure of the IV-SOPRA subscales. Each subscale demonstrates good to excellent fit to the data, according to fit measures and reliability indices. The instrument provides Higher Education institutions with a tool for evaluating and diagnosing levels of prejudice and bullying on campus, so that effective measures can be put in place to deal with problems at hand.