The objective of this study was to explore the factors of professional readiness in the healthcare field of athletic training. We used a qualitative research design based in grounded theory. Participants included newly certified athletic trainers and athletic training supervisors from collegiate, secondary school, and emerging work settings. After completing the demographic questionnaire, 14 newly certified athletic trainers and 9 supervisors were selected to complete one-on-one interviews using GoToMeeting audiovisual web conferencing software. Data was analyzed using a constant comparative analysis as well as open and axial coding. We established trustworthiness using bracketing interviews, reflexivity, triangulation, member checks, and peer reviews. Analysis revealed four overarching themes including organization and administration, interpersonal relations, athletic training attributes, and confidence. Readiness for autonomous practice is multifaceted and exceeds clinical skills. Athletic trainers must possess certain personality characteristics and confidence, as well as interpersonal, organizational, and clinical skills that can be incorporated into daily practice.