We aimed to tackle the question about the time course of plausibility effect in on-line processing of Chinese nouns in temporarily ambiguous structures, and whether L2ers can immediately use the plausibility information generated from classifier-noun associations in analyzing ambiguous structures. Two eye-tracking experiments were conducted to explore how native Chinese speakers (Experiment 1) and high-proficiency Dutch-Chinese learners (Experiment 2) on-line process 4-character novel noun-noun combinations in Chinese. In each pair of nominal phrases (Numeral+Classifier+Noun1+Noun2), the plausibility of Classifier-Noun1 varied (plausible vs. implausible) while the whole nominal phrases were always plausible. Results showed that the plausibility of Classifier-Noun1 associations had an immediate effect on Noun1, and a reversed effect on Noun2 for both groups of participants. These findings indicated that plausibility plays an immediate role in incremental semantic integration during on-line processing of Chinese. Similar to native Chinese speakers, high-proficiency L2ers can also use the plausibility information of classifier-noun associations in syntactic reanalysis.