This study examines the impact of inclusion on secondary students by focusing on the performance of students without disabilities in the inclusive environment compared to their performance in a segregated environment. Many studies exist demonstrating the positive impact of the inclusive environment on the performance of students with disabilities. However, there is little research demonstrating the impact of the inclusive environment on the performance of students without disabilities. This randomized control experiment showed that there was no significant difference in the performance of students without disabilities in the inclusive versus segregated environments in reading, science, and social studies content areas. Students without disabilities scored significantly higher in segregated environments versus inclusive environments in math. Further research examining the impact of the environment on the performance of students without disabilities is recommended.