Bacteria have developed a cell-to-cell communication system called quorum sensing (QS), allowing them to regulate group behavior and synchronize the expression of virulence factors, responsible for increasing their infection capacity and resistance to antimicrobials. Although the control of microbial infections through the inhibition of microbial…

Owing to the critical role of stereochemistry in biotechnology, medicine, and industry, it must be well represented in undergraduate lab curricula. To achieve these stereochemical laboratory requirements without sacrificing existing lab techniques, we modified two standard laboratory experiments to include chiral GC analysis as follows. (1) The…

When structure-property relationships are discussed in inorganic chemistry and materials science, the common perception is that materials with the same composition and atomic arrangement have the same properties. In this laboratory experiment, we show that "processing"--the exact conditions under which materials are made--can in fact…

Teaching graduate students the entanglements of the interdisciplinary field of electrochemical DNA biosensors in microfluidics can be challenging, as it involves multiple fields of research strongly interacting with each other. Yet, electrochemical biosensors coupled with the improved hydrodynamics of microfluidics allow for simpler, more…

Lakes are artists' pigments made from colorants bound to an inert substrate, usually hydrated aluminum oxide. Before the late 19th century, lakes were made from natural pigments extracted from plants and insects, extracted either directly from the dyestuff or indirectly from waste materials generated in the manufacture of dyed textiles. The first…

Natural sciences can be difficult to grasp because physical and chemical phenomena can take place across time and length scales that are beyond the reach of human perception. This problem is particularly true for students attempting to learn about microfluidics, a discipline that involves intricate engineering methods and fluid phenomena that are…

As an introduction to quadrupolar effects in NMR spectroscopy, students use low field ([superscript 1]H, 60 MHz), benchtop [superscript 13]C NMR spectroscopy to contrast the spin-spin coupling behavior of [superscript 13]C to the dipolar [superscript 1]H and quadrupolar [superscript 2]D in familiar solvents C(H/D)Cl[subscript 3], C(H/D)[subscript…

A one-pot multicomponent synthesis of unsymmetrical indole-substituted methane derivative based on two consecutive reactions of p-methylbenzaldehyde with N-methylindole and 5,5-dimethyl-1,3-cyclohexanedione is described as an experiment that was carried out by second-year undergraduate chemistry students individually in a laboratory classroom. The…

An effective, practical approach for incorporating molecular biology tools to biology and biochemistry students is described. For 6 weeks, students are exposed to bacterial transformation, plasmid DNA purification and analysis, parasite transfection, ectopic expression of fluorescent proteins, fluorescence microscopy, and flow cytometry analysis.…

Understanding potentiometry is a difficult task when students in initial courses of Analytical Chemistry are first introduced to instrumental techniques. The closest instrument to them is a pH meter, which is frequently used as a "black box", without a real understanding of how it works. Here we present the assembly of jelly modules that…

This laboratory experiment offers a research-style experience designed for senior undergraduate students in a multidisciplinary laboratory setting. The experiment focuses on the synthesis, characterization, and application of a cation-exchange membrane made of COF-SO[subscript 3]Na/PAN for ion separation. By participating in this practical…

Ultrafast laser pump-probe spectroscopy is an important and growing field of physical chemistry that allows the measurement of chemical dynamics on their natural time scales, but undergraduate laboratory courses lack examples of such spectroscopy and the interpretation of the dynamics that occur. Here we develop and implement an ultrafast…

Chemists have always designed and deployed cross-disciplinary approaches that interface chemistry with other disciplines to proffer solutions to many human problems. We designed a laboratory experiment that implements chemistry concepts in a biological model to prime undergraduate students to appreciate interdisciplinarity as an approach to…

Many of the current synthetic methodologies utilized within academic and industrial laboratories require knowledge of how to safely handle air- and moisture-sensitive reagents and work under inert atmospheres. As a result, the ACS Committee on Professional Training recommends incorporating synthetic methods that make use of these inert atmospheres…

This report outlines an approach for preparing 5-color 3D printed plastic models of molecular orbitals and electron density surfaces using a hobby-grade 3D printer. Instructions are provided for preparing 3D orbital and electron density surface (EDS) models using solid or mesh representations in ground state and transition state structures. We…