The COVID-19 pandemic stimulated education system worldwide to employ online learning to support learning despite difficult times. To respond to this challenge and to promote Sustainable Development Goal (SDG) 4, advocating quality education, a virtual learning material (VLM) for Biology was articulated in Google Classroom. Accordingly, this study aimed to evaluate its acceptability and conformity to the international standards for online courses using the Open SUNY Course Quality Review (OSCQR) rubric as the questionnaire. Respondents (N=40) involved four stakeholders: Senior High School Students, Pre-service Science Teachers, High School Teachers, and Science Instructors/Professors, with n=10 representatives each group. Their perspectives of the VLM acceptability in terms of Overview and Information, Technology and Tool, Design and Layout, Content and Activities, Interaction, and Assessment and Feedback were obtained through a Google Form by rating the 50-item questionnaire on a 4-point Likert scale together with two open-ended questions. With a grand mean of 3.81(SD=0.40), the findings revealed highly acceptable results. The qualitative responses also substantiated this result. Significant differences in the responses are also discussed, while the Cronbach alpha reliability test is high ([alpha]=0.923). Significantly, the VLM conforms with the international standards for online course design, suggesting it can be implemented among target students.