This study focuses on the effect of the programme variable on the purposes and effects that students associate with assessment, on the assessment methods used and on the perceived use of assessment. Data were collected in five Portuguese Public Universities through a survey (n = 4144) and focus group (n = 250) with students enrolled in different programmes. Findings point to statistically significant differences in relation to the purpose of assessment, assessment methods most used and perceived use of assessment. The main differences were found in the kinds of methods used in different programmes: Law reported the lowest frequency of the use of collective assessment methods and portfolios, whereas Psychology, Mechanical and Industrial Engineering were the programmes that reported the lowest frequency of use of individual methods. Educational sciences reported more frequency of all types of methods and reported significantly more preference for the use of alternative methods than the remaining programmes. Negative emotions were most associated with assessment by Nursing students and Educational Sciences' students reported more participation in the assessment process than students from all other programmes. Implications of the findings are discussed.