The purpose of the present study was to examine the effect of acute Whole Body Vibration (WBV) on static and dynamic balance in physical education students. A total of 30 healthy physical education students participated in this study. The participants were divided into two groups as the Control Group (n = 15) and the Whole Body Vibration group (n = 15). The control group did not carry out any trainings; and the WBV group did acute WBV. The first measurements of height, weight, flamingo balance test for static balance, and the Y balance test for dynamic balance were made for both groups before applying the WBV program. The final measurements were made right after the WBV application. Results are presented as mean ± standard deviation. The values that were obtained from the pre-test and post-test were analyzed with the paired sample t-test after their normality was checked with the Shapire-Wilk Test. The comparisons between the groups were analyzed by using an independent sample t-test at a significance level of p < 0.05. The flamingo balance test was applied to determine the static balance values. According to the flamingo balance test, there was a significant difference between the right leg pre-test and post-test values of the WBV group (p < 0.05). A significant difference was detected between the left leg pre-test and post-test values of the WBV group (p < 0.05). The Y balance test was applied to determine the dynamic balance results. There was a significant difference between the right leg pre-test and post-test values of the WBV group (p < 0.05). There was a significant difference between the left leg pre-test and post-test values of the WBV group. It was determined that the acute WBV has a positive effect on static and dynamic balance.