Objective: This study compared self-reported nutrition, physical activity, and body mass index (BMI) in undergraduate versus graduate nursing students. Participants: Respondents included 233 undergraduate and 230 graduate nursing students in a Southeastern public university. Methods: The study was an exploratory, descriptive, cross-sectional comparison administered via online questionnaire including self-reported demographics, nutritional intake, physical activity, and BMI. Results: Undergraduates reported exercising more than graduate students. Graduate students reported consuming significantly more vegetables than undergraduates; however, more graduate students self-reported BMIs in overweight and obese categories than undergraduates. Conclusions: Findings provide a foundational understanding that interventions or educational programs to improve self-care behaviors of nursing students should be tailored depending on the educational level of the students. Educators must establish self-care skills within nursing students at all levels to help them preserve their health in the fast-paced, strenuous, and stressful work of all echelons of nursing upon completion of the educational program.